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Bio-Rad lav blot 1 kit
Lav Blot 1 Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lav+blot+1+kit/Western+Blot+Temperature+Sensitive+Reagents+Bag/pmc02726149-101-32-36
Average 90 stars, based on 1 article reviews

lav blot 1 kit - by Bioz Stars, 2026-09

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Article Title: Performance of the Liaison XL Murex HIV Ab/Ag Test on Clinical Samples Representing Current Epidemic HIV Variants: TABLE 1
Article Snippet: .. Complementary serological assays were performed, including the Vidas HIV Duo Quick (bioMérieux) and the Murex HIV Ag/Ab combination (DiaSorin), both of which detect antigens and antibodies, and Western blotting with the New LAV BLOT 1 kit and New LAV BLOT 2 kit (Bio-Rad) and Vidas HIV P24 II (bioMérieux) to detect the p24 antigen of HIV-1. .. Molecular tests were also carried out on each discordant sample to identify a possible early step of primary infection: the viral load was assessed by the Abbott RealTime HIV-1 assay, and the RT and protease genes of HIV-1 group M were examined by reverse transcription (RT)-PCR when sufficient volume was available (15).

Article Title: Prevalence and risk factors for Hepatitis C and HIV-1 infections among pregnant women in Central Brazil
Article Snippet: Screened women with positive and indeterminate results were re-tested using sera from venous puncture blood samples (GENSCREEN ® HIV1/2 kit Bio-Rad, France) that recognizes antibodies against antigens gp160 and gp25 or by HIV-1/2 ELISA test (Wiener lab., Argentina) that detects antibodies against the pop and gag regions' antigens. .. In parallel, at least one of the following confirmatory tests were applied: Indirect Immunofluorecence by IFI – HIV1 kit (Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro); Immuno Blot (IB) using a NEW LAV BLOT 1 kit (Bio-Rad, France) that detects antibodies against HIV-1 gp160, gp110/120, p68, p55, p52, gp41, p40, p34, p24/25 and p18; Western Blot (WB) for detection of HIV1 and HIV2, using HIV BLOT 2.2 kit (Genelabs ® Diagnostics Pte Ltd, Singapore) and qualitative "Nested" PCR to detect pro viral HIV1 DNA in blood mononuclear cells (50 copies/mL detection limit), performed at the Center of Genomics Laboratory (São Paulo, Brazil). ..

Article Title: Prime-boost vaccination with plasmid DNA and a chimeric adenovirus type 5 vector with type 35 fiber induces protective immunity against HIV.
Article Snippet: .. By Western blotting method, the HIV envelope glycoprotein gp160-coated membrane from the New Lav Blot 1 kit (Bio-Rad, Marnes-la-Coquette, France) was incubated with a 100-fold dilution of mouse serum followed by an affinity-purified horseradish peroxidase (HRP)-labeled anti-mouse immunoglobulin (ICN Pharmaceuticals Inc., OH, USA). .. HIV gp160 protein was detected using the ECL Plus Western Blotting Detection System (Amersham Pharmacia Biotech).

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The HIV-1 subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.

Journal: Virology Journal

Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

doi: 10.1186/1743-422X-6-87

Figure Lengend Snippet: The HIV-1 subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.

Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

Techniques: Expressing, Plasmid Preparation, Recombinant, SDS Page

HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by IFN-γ and IL-4 ELISPOT assays . Groups of mice (5 mice per group) were vaccinated three times (day 0, 28 and 56) with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM). Mice were sacrificed on day 84, and splenocytes were prepared from isolated spleens pooled from 5 mice per group then used in IFN-γ (A) and IL-4 (B) ELISPOT assays with R10 medium only (negative assay control) or with the Gag CD4 peptide. Bars are the mean number of spots from triplicate wells and are expressed as SFU/10e6 splenocytes. Differences in immune responses between vaccine groups were analyzed by a two-sample student's t-test. A p < 0.05 was considered statistically significant. The differences in response between stimulated cells and unstimulated cells within the same vaccine group were also analyzed by a student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

Journal: Virology Journal

Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

doi: 10.1186/1743-422X-6-87

Figure Lengend Snippet: HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by IFN-γ and IL-4 ELISPOT assays . Groups of mice (5 mice per group) were vaccinated three times (day 0, 28 and 56) with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM). Mice were sacrificed on day 84, and splenocytes were prepared from isolated spleens pooled from 5 mice per group then used in IFN-γ (A) and IL-4 (B) ELISPOT assays with R10 medium only (negative assay control) or with the Gag CD4 peptide. Bars are the mean number of spots from triplicate wells and are expressed as SFU/10e6 splenocytes. Differences in immune responses between vaccine groups were analyzed by a two-sample student's t-test. A p < 0.05 was considered statistically significant. The differences in response between stimulated cells and unstimulated cells within the same vaccine group were also analyzed by a student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

Techniques: Enzyme-linked Immunospot, Recombinant, Isolation

HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by the amount of cytokines secreted by stimulated cells . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Splenocytes isolated and pooled from 5 mice per group on day 84 were incubated in R10 medium only (negative assay control), or stimulated with a Gag CD4 peptide for 48 hrs. Cytokines released into the supernatant were quantified using a mouse Th1/Th2 cytokine bead array (CBA) assay. (A) IFN-γ, (B) TNF-α, (C) IL-4 and (D) IL-5. Each bar in the graphs represents the average picogram amount of cytokine produced per 10e6 splenocytes in 48 hours of stimulation with media or Gag CD4 peptide, for triplicate responses. A p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

Journal: Virology Journal

Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

doi: 10.1186/1743-422X-6-87

Figure Lengend Snippet: HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by the amount of cytokines secreted by stimulated cells . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Splenocytes isolated and pooled from 5 mice per group on day 84 were incubated in R10 medium only (negative assay control), or stimulated with a Gag CD4 peptide for 48 hrs. Cytokines released into the supernatant were quantified using a mouse Th1/Th2 cytokine bead array (CBA) assay. (A) IFN-γ, (B) TNF-α, (C) IL-4 and (D) IL-5. Each bar in the graphs represents the average picogram amount of cytokine produced per 10e6 splenocytes in 48 hours of stimulation with media or Gag CD4 peptide, for triplicate responses. A p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

Techniques: Recombinant, Isolation, Incubation, Produced

HIV-1 subtype C Gag-specific serum IgG responses in mice vaccinated with recombinant Salmonella vaccine vector . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Serum (pooled from 5 mice per group) was isolated from blood taken before each vaccination on day 0, 28 and 56 and just before sacrifice on day 84. (A) The HIV-1 Gag-specific IgG for each group of mice with a 1/100 serum dilution. The data are the ratio of the OD 405 nm for vaccinated mice and the OD 405 nm for the day 0 serum (pre-bleed). (B) The HIV-1 Gag-specific IgG1 and IgG2a were measured in serum of each group of mice on Day 84 with a 1/100 serum dilution. Each bar represents the mean OD 405 nm value. Differences in antibody responses between vaccine groups at different time points were analyzed by a two-sample student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

Journal: Virology Journal

Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

doi: 10.1186/1743-422X-6-87

Figure Lengend Snippet: HIV-1 subtype C Gag-specific serum IgG responses in mice vaccinated with recombinant Salmonella vaccine vector . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Serum (pooled from 5 mice per group) was isolated from blood taken before each vaccination on day 0, 28 and 56 and just before sacrifice on day 84. (A) The HIV-1 Gag-specific IgG for each group of mice with a 1/100 serum dilution. The data are the ratio of the OD 405 nm for vaccinated mice and the OD 405 nm for the day 0 serum (pre-bleed). (B) The HIV-1 Gag-specific IgG1 and IgG2a were measured in serum of each group of mice on Day 84 with a 1/100 serum dilution. Each bar represents the mean OD 405 nm value. Differences in antibody responses between vaccine groups at different time points were analyzed by a two-sample student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

Techniques: Recombinant, Plasmid Preparation, Isolation

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